NAT10 Antibody (5Z3E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15742

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15742-100ul
NBP3-15742-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Z3E5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NAT10 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 750-1025 of human NAT10 (Q9H0A0). KTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NAT10 Antibody (5Z3E5)

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742]

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742]

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742] - Analysis of extracts of various cell lines, using NAT10 antibody (NBP3-15742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry-Paraffin: NAT10 Antibody (5Z3E5) [NBP3-15742]

Immunohistochemistry-Paraffin: NAT10 Antibody (5Z3E5) [NBP3-15742]

Immunohistochemistry-Paraffin: NAT10 Antibody (5Z3E5) [NBP3-15742] - Mouse pancreas using NAT10 Rabbit mAb (NBP3-15742) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742]

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742]

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742] - Analysis of extracts of Mouse testis, using NAT10 antibody (NBP3-15742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Applications for NAT10 Antibody (5Z3E5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NAT10

NAT10 has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, a

Long Name

RNA cytidine acetyltransferase

Alternate Names

ALP, EC 2.3.1.-, hALP, KIAA1709

Gene Symbol

NAT10

Additional NAT10 Products

Product Documents for NAT10 Antibody (5Z3E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NAT10 Antibody (5Z3E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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