NCAPD3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1573
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1450 and the C-terminus (residue 1507) of human Chromosome-Associated Protein D3 using the numbering given in entry NP_056076.1 (GeneID 23310).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NCAPD3 Antibody
![Western Blot: NCAPD3 Antibody [NB100-1573]](https://resources.bio-techne.com/images/products/NCAPD3-Antibody-NB100-1573-img0001.jpg "Western Blot: NCAPD3 Antibody [NB100-1573]")
Western Blot: NCAPD3 Antibody [NB100-1573]
Western Blot: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-D3 antibody NB100-1573 (lotNB100-1573-4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: NCAPD3 Antibody [NB100-1573]](https://resources.bio-techne.com/images/products/NCAPD3-Antibody-NB100-1573-img0002.jpg "Immunoprecipitation: NCAPD3 Antibody [NB100-1573]")
Immunoprecipitation: NCAPD3 Antibody [NB100-1573]
Immunoprecipitation: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody (lot NB100-1573-4) used for IP at 6 ug per reaction. CAP-D3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1573-3). For blotting immunoprecipitated CAP-D3, NB100-1573 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Applications for NCAPD3 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30455981).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NCAPD3
Alternate Names
CAPD3, CAP-D3, FLJ42888, hCAP-D3condensin-2 complex subunit D3, hcp-6, hHCP-6, KIAA0056MGC104671, Non-SMC condensin II complex subunit D3, non-SMC condensin II complex, subunit D3
Entrez Gene IDs
23310 (Human)
Gene Symbol
NCAPD3
UniProt
Additional NCAPD3 Products
Product Documents for NCAPD3 Antibody
Product Specific Notices for NCAPD3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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