NCAPG2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1813
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1143) of human Chromosome-Associated Protein G2 using the numbering given in entry AAH43404.1 (GeneID 54892).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NCAPG2 Antibody
![Western Blot: NCAPG2 Antibody [NB100-1813]](https://resources.bio-techne.com/images/products/NCAPG2-Antibody-Western-Blot-NB100-1813-img0004.jpg "Western Blot: NCAPG2 Antibody [NB100-1813]")
Western Blot: NCAPG2 Antibody [NB100-1813]
Western Blot: NCAPG2 Antibody [NB100-1813] - Detection of Human CAP-G2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody NB100-1813 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: NCAPG2 Antibody [NB100-1813]](https://resources.bio-techne.com/images/products/NCAPG2-Antibody-Immunoprecipitation-NB100-1813-img0005.jpg "Immunoprecipitation: NCAPG2 Antibody [NB100-1813]")
Immunoprecipitation: NCAPG2 Antibody [NB100-1813]
Immunoprecipitation: NCAPG2 Antibody [NB100-1813] - Detection of human CAP-G2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody NB100-1813 used for IP at 6 ug per reaction. CAP-G2 was also immunoprecipitated by another rabbit anti-CAP-G2 antibody. For blotting immunoprecipitated CAP-G2, NB100-1813 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for NCAPG2 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg of lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30143891). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NCAPG2
Alternate Names
Chromosome-associated protein G2, FLJ20311, Leucine zipper protein 5CAP-G2hCAP-G2condensin-2 complex subunit G2, LUZP5, more than blood homolog, MTB, Non-SMC condensin II complex subunit G2, non-SMC condensin II complex, subunit G2
Entrez Gene IDs
54892 (Human)
Gene Symbol
NCAPG2
UniProt
Additional NCAPG2 Products
Product Documents for NCAPG2 Antibody
Product Specific Notices for NCAPG2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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