Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NCAPH2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 425 and 475 of human chromosome-associated protein H2 using the numbering given in entry NP_689512.2 (GeneID 29781).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCAPH2 Antibody

Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723] - Detection of Human CapH2 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and (50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CapH2 antibody NBP1-28723 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry: NCAPH2 Antibody [NBP1-28723] - Detection of human CAP-H2 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CAP-H2 (NBP1-28723). Detection: DAB
Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723] - Detection of Human CAP-H2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CAP-H2 antibody NBP1-28723 used for WB at 1 mcg/ml (A and B) and used for IP at 10 mcg/mg lysate. CAP-H2 was also immunoprecipitated by rabbit anti-CAP-H2 antibody NBP1-28682, which recognizes a downstream epitope. For blotting immunoprecipitated CapH2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
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Applications for NCAPH2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCAPH2

CAP-H2 (chromosome-associated protein H2) is 1 of 3 non-SMC subunits that define the condensin II complex. Condensin II along with the condensin I plays a critical role in mitotic chromosome assembly and segregation.

Alternate Names

CAP-H2, CAP-H2 subunit of the condensin II complex, CAPH2,384D8-2, Chromosome-associated protein H2, condensin-2 complex subunit H2, hCAP-H2, kleisin beta, Kleisin-beta, MGC15858, MGC18000, MGC2455, MGC4133, MGC5305, MGC8640, Non-SMC condensin II complex subunit H2, non-SMC condensin II complex, subunit H2

Entrez Gene IDs

29781 (Human)

Gene Symbol

NCAPH2

UniProt

NP_689512.2 (Human)

Additional NCAPH2 Products

Product Documents for NCAPH2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NCAPH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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