NCAPH2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49580

Novus Biologicals, part of Bio-Techne
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NBP2-49580
NBP2-49580-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCAPH2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EAENEFLSLDDFPDSRTNVDLKNDQTPSEVLIIPLLPMALVAPDEMEKNNNPLYSRQGEVLASRKDFRMNTCVPHPRGAFMLEPEGMS

Reactivity Notes

Mouse (81%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCAPH2 Antibody

Immunohistochemistry-Paraffin: NCAPH2 Antibody [NBP2-49580]

Immunohistochemistry-Paraffin: NCAPH2 Antibody [NBP2-49580]

Immunohistochemistry-Paraffin: NCAPH2 Antibody [NBP2-49580] - Staining in human bone marrow and pancreas tissues using anti-NCAPH2 antibody. Corresponding NCAPH2 RNA-seq data are presented for the same tissues.
Western Blot: NCAPH2 Antibody [NBP2-49580]

Western Blot: NCAPH2 Antibody [NBP2-49580]

Western Blot: NCAPH2 Antibody [NBP2-49580] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: NCAPH2 Antibody [NBP2-49580]

Immunocytochemistry/ Immunofluorescence: NCAPH2 Antibody [NBP2-49580]

Immunocytochemistry/Immunofluorescence: NCAPH2 Antibody [NBP2-49580] - Staining of human cell line MCF7 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.
View 2 more images

Applications for NCAPH2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCAPH2

CAP-H2 (chromosome-associated protein H2) is 1 of 3 non-SMC subunits that define the condensin II complex. Condensin II along with the condensin I plays a critical role in mitotic chromosome assembly and segregation.

Alternate Names

CAP-H2, CAP-H2 subunit of the condensin II complex, CAPH2,384D8-2, Chromosome-associated protein H2, condensin-2 complex subunit H2, hCAP-H2, kleisin beta, Kleisin-beta, MGC15858, MGC18000, MGC2455, MGC4133, MGC5305, MGC8640, Non-SMC condensin II complex subunit H2, non-SMC condensin II complex, subunit H2

Gene Symbol

NCAPH2

Additional NCAPH2 Products

Product Documents for NCAPH2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCAPH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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