NCBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33897

Novus Biologicals, part of Bio-Techne
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NBP2-33897
NBP2-33897-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCBP1 Antibodies »

Product Summary for NCBP1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCBP1 Antibody

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897]

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897]

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897] - Staining in human testis and pancreas tissues using anti-NCBP1 antibody. Corresponding NCBP1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: NCBP1 Antibody [NBP2-33897]

Immunocytochemistry/ Immunofluorescence: NCBP1 Antibody [NBP2-33897]

Immunocytochemistry/Immunofluorescence: NCBP1 Antibody [NBP2-33897] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897]

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897]

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897] - Staining of human pancreas shows low expression as expected.
View 2 more images

Applications for NCBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCBP1

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq]

Alternate Names

CBP80nuclear cap binding protein subunit 1, 80kD, NCBP 80 kDa subunit, NCBPMGC2087, nuclear cap binding protein subunit 1, 80kDa, nuclear cap-binding protein subunit 1,80 kDa nuclear cap-binding protein, Sto1

Gene Symbol

NCBP1

UniProt

Q09161 (Human)

Additional NCBP1 Products

Product Documents for NCBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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