NCF1 Antibody (A6B10-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32696
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A6B10-R
Concentration
1 mg/ml
Product Summary for NCF1 Antibody (A6B10-R)
Immunogen
Recombinant protein within human NCF1 aa 51-250. (Uniprot: P14598)
Localization
Cytoplasm, cytosol, Membrane.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NCF1 Antibody (A6B10-R)
Western Blot: NCF1 Antibody (A6B10-R) [NBP3-32696]
Western Blot: NCF1 Antibody (A6B10-R) [NBP3-32696] - Western blot analysis of NCF1 on different lysates with Mouse anti-NCF1 antibody (NBP3-32696) at 1/2,000 dilution.Lane 1: Ramos cell lysate
Lane 2: Raji cell lysate
Lane 3: Daudi cell lysate
Lane 4: HL-60 cell lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32696) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for NCF1 Antibody (A6B10-R)
Application
Recommended Usage
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NCF1
Alternate Names
47 kDa neutrophil oxidase factor, FLJ79451, NADPH oxidase organizer 2, NCF-1, NCF1A, neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein, neutrophil cytosolic factor 1, neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1), neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1), Neutrophil NADPH oxidase factor 1, Nox organizer 2, NOXO2NCF-47K, Nox-organizing protein 2, p47phox, SH3 and PX domain-containing protein 1A, SH3PXD1Ap47-phox
Gene Symbol
NCF1
Additional NCF1 Products
Product Documents for NCF1 Antibody (A6B10-R)
Product Specific Notices for NCF1 Antibody (A6B10-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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