NCF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19486
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for NCF1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NCF1. The exact sequence is proprietary.
Reactivity Notes
Mouse (83%), Rat (80%), Pig (82%), Rabbit (86%), Bovine (87%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NCF1 Antibody
![Western Blot: NCF1 Antibody [NBP2-19486]](https://resources.bio-techne.com/images/products/NCF1-Antibody-Western-Blot-NBP2-19486-img0001.jpg "Western Blot: NCF1 Antibody [NBP2-19486]")
Western Blot: NCF1 Antibody [NBP2-19486]
Western Blot: NCF1 Antibody [NBP2-19486] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:5000.![Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-19486]](https://resources.bio-techne.com/images/products/NCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19486-img0005.jpg "Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-19486]")
Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-19486]
Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-19486] - Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NCF1 protein stained by NCF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-19486]](https://resources.bio-techne.com/images/products/NCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-19486-img0002.jpg "Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-19486]")
Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-19486]
Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-19486] - Hela xenograft, using NCF1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for NCF1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NCF1
Alternate Names
47 kDa neutrophil oxidase factor, FLJ79451, NADPH oxidase organizer 2, NCF-1, NCF1A, neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein, neutrophil cytosolic factor 1, neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1), neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1), Neutrophil NADPH oxidase factor 1, Nox organizer 2, NOXO2NCF-47K, Nox-organizing protein 2, p47phox, SH3 and PX domain-containing protein 1A, SH3PXD1Ap47-phox
Gene Symbol
NCF1
UniProt
Additional NCF1 Products
Product Documents for NCF1 Antibody
Product Specific Notices for NCF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.