NCF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26113
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DEAEDPDPNYAGEP corresponding to internal region according to NP_035006.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NCF1 Antibody
![Western Blot: NCF1 Antibody [NBP2-26113]](https://resources.bio-techne.com/images/products/NCF1-Antibody-Western-Blot-NBP2-26113-img0001.jpg "Western Blot: NCF1 Antibody [NBP2-26113]")
Western Blot: NCF1 Antibody [NBP2-26113]
Western Blot: NCF1 Antibody [NBP2-26113] - Staining of Daudi cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]](https://resources.bio-techne.com/images/products/NCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26113-img0003.jpg "Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]")
Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]
Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]](https://resources.bio-techne.com/images/products/NCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26113-img0002.jpg "Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]")
Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]
Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for NCF1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:2000
Western Blot
1 - 3 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NCF1
Alternate Names
47 kDa neutrophil oxidase factor, FLJ79451, NADPH oxidase organizer 2, NCF-1, NCF1A, neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein, neutrophil cytosolic factor 1, neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1), neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1), Neutrophil NADPH oxidase factor 1, Nox organizer 2, NOXO2NCF-47K, Nox-organizing protein 2, p47phox, SH3 and PX domain-containing protein 1A, SH3PXD1Ap47-phox
Gene Symbol
NCF1
Additional NCF1 Products
Product Documents for NCF1 Antibody
Product Specific Notices for NCF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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