NCF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61666

Novus Biologicals, part of Bio-Techne
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NB100-61666
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NCF4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KDFPEEDDPTN corresponding to internal region according to NP_000622.2, NP_038202.1.

Epitope

KDFPEEDDPTN

Specificity

This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NCF4 Antibody

Western Blot: NCF4 Antibody [NB100-61666]

Western Blot: NCF4 Antibody [NB100-61666]

Western Blot: NCF4 Antibody [NB100-61666] - HEK293 overexpressing P40PHOX and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666]

Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666]

Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666] - Staining of paraffin embedded Human Spleen at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NCF4 Antibody [NB100-61666]

Western Blot: NCF4 Antibody [NB100-61666]

Western Blot: NCF4 Antibody [NB100-61666] - Analysis of NCF4 antibody using NB100-61666 at 0.01ug/ml in Daudi cell lysate (35ug protein in RIPA buffer).
View 1 more images

Applications for NCF4 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in Daudi lysates (calculated MW of 39.0 kDa band according to NP_000622.2 and 38.8 kDa band according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human tonsil shows staining of the cytoplasm in some cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCF4

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Alternate Names

MGC3810, NCF, NCF-4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD), neutrophil cytosolic factor 4, 40kDa, Neutrophil NADPH oxidase factor 4, p40phox, p40-phox, SH3 and PX domain-containing protein 4, SH3PXD4P40PHOX

Entrez Gene IDs

4689 (Human)

Gene Symbol

NCF4

UniProt

NP_000622.2 (Human)

Additional NCF4 Products

Product Documents for NCF4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NCF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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