NCOA3/AIB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-567

Novus Biologicals, part of Bio-Techne
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NB300-567
NB300-567-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Xenopus (92%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all NCOA3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues G(3) L G E N L D P L A S D S(15) C of human AIB1.

Localization

Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

Specificity

Detects amplified in breast cancer 1 (nuclear receptor coactivator 3) from human tissues and cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA3/AIB1 Antibody

Western Blot: NCOA3/AIB1 Antibody [NB300-567]

Western Blot: NCOA3/AIB1 Antibody [NB300-567]

Western Blot: NCOA3/AIB1 Antibody [NB300-567] - Analysis of human AIB1 in T47D cell lysate.

Applications for NCOA3/AIB1 Antibody

Application
Recommended Usage

Western Blot

2 ug/ml
Application Notes
WB: Predicted molecular weight: 155 kDa. Can be blocked with AIB 1 peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCOA3

NCOA3/SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. NCOA3/SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. NCOA3/SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. NCOA3/SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. NCOA3/SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Entrez Gene IDs

116833 (Human)

Gene Symbol

NCOA3

Additional NCOA3 Products

Product Documents for NCOA3/AIB1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NCOA3/AIB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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