NCOA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2571

Novus Biologicals, part of Bio-Techne
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NB100-2571
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NCOA5 Antibodies »

Product Summary for NCOA5 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 525 and the C-terminus (residue 579) of human Nuclear Receptor Coactivator 5 (Coactivator Independent of AF-2) using the numbering given in entry NP_066018.1 (GeneID 57727).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NCOA5 Antibody

Western Blot: NCOA5 Antibody [NB100-2571]

Western Blot: NCOA5 Antibody [NB100-2571]

Western Blot: NCOA5 Antibody [NB100-2571] - Detection of Human and Mouse NCOA5 (CIA) on HeLa whole cell lysate using NB100-2571. NCOA5 (CIA) was also immunoprecipitated by rabbit anti-NCOA5 (CIA) antibody NB100-2570.
Immunohistochemistry-Paraffin: NCOA5 Antibody [NB100-2571]

Immunohistochemistry-Paraffin: NCOA5 Antibody [NB100-2571]

Immunohistochemistry-Paraffin: NCOA5 Antibody [NB100-2571] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NCOA5 (CIA) used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for NCOA5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA5

NCOA5 is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c-myc in response to 17-beta-estradiol (E2).

Alternate Names

CIAbA465L10.6, Coactivator independent of AF-2, KIAA1637, NCoA-5, nuclear receptor coactivator 5

Entrez Gene IDs

57727 (Human); 228869 (Mouse)

Gene Symbol

NCOA5

UniProt

Q9HCD5 (Human)

Additional NCOA5 Products

Product Documents for NCOA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCOA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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