NCOA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47361

Novus Biologicals, part of Bio-Techne
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NBP2-47361
NBP2-47361-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCOA5 Antibodies »

Product Summary for NCOA5 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NECREKEREEIARQAAKMADEAILQERERGGPEEGVRGGHPPAIQSLINLLADNRYLTAEETDKIINYLRERKERLMRSSTDSLPGPISRQPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA5 Antibody

Immunocytochemistry/ Immunofluorescence: NCOA5 Antibody [NBP2-47361]

Immunocytochemistry/ Immunofluorescence: NCOA5 Antibody [NBP2-47361]

Immunocytochemistry/Immunofluorescence: NCOA5 Antibody [NBP2-47361] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & actin filaments.
Immunohistochemistry-Paraffin: NCOA5 Antibody [NBP2-47361]

Immunohistochemistry-Paraffin: NCOA5 Antibody [NBP2-47361]

Immunohistochemistry-Paraffin: NCOA5 Antibody [NBP2-47361] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
NCOA5 Antibody

Western Blot: NCOA5 Antibody [NBP2-47361] -

Western Blot: NCOA5 Antibody [NBP2-47361] -Analysis in human cell line SCLC-21H.
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Applications for NCOA5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCOA5

NCOA5 is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c-myc in response to 17-beta-estradiol (E2).

Alternate Names

CIAbA465L10.6, Coactivator independent of AF-2, KIAA1637, NCoA-5, nuclear receptor coactivator 5

Gene Symbol

NCOA5

Additional NCOA5 Products

Product Documents for NCOA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCOA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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