ND6 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94464

Novus Biologicals, part of Bio-Techne
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NBP2-94464-0.1ml
NBP2-94464-0.02ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ND6 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 80-174 of human ND6 (YP_003024037.1). LIREDPIGAGALYDYGR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ND6 Antibody - Azide and BSA Free

Western Blot: ND6 AntibodyAzide and BSA Free [NBP2-94464]

Western Blot: ND6 AntibodyAzide and BSA Free [NBP2-94464]

Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.
Immunohistochemistry-Paraffin: ND6 Antibody - Azide and BSA Free [NBP2-94464]

Immunohistochemistry-Paraffin: ND6 Antibody - Azide and BSA Free [NBP2-94464]

Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Rat kidney using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: ND6 AntibodyAzide and BSA Free [NBP2-94464]

Western Blot: ND6 AntibodyAzide and BSA Free [NBP2-94464]

Western Blot: ND6 Antibody [NBP2-94464] - Analysis of HepG2, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
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Applications for ND6 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ND6

Alternate Names

mitochondrially encoded NADH dehydrogenase 6, MTND6, MT-ND6, NADH dehydrogenase subunit 6, NADH dehydrogenase, subunit 6 (complex I), NADH6

Gene Symbol

ND6

Additional ND6 Products

Product Documents for ND6 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ND6 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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