NDRG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72052

Novus Biologicals, part of Bio-Techne
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NBP1-72052
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NDRG2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KEAELAARILLDQGQ-C corresponding to N-Terminus (near) according to NP_963293.1.

Reactivity Notes

Expected from sequence similarity: Canine

Specificity

This antibody is expected to recognize reported isoform a (NP_963293.1) only. Reported variants represent identical protein: NP_963831.1, NP_963293.1, NP_963834.1, NP_963833.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NDRG2 Antibody

Western Blot: NDRG2 Antibody [NBP1-72052]

Western Blot: NDRG2 Antibody [NBP1-72052]

Western Blot: NDRG2 Antibody [NBP1-72052] - (0.1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-72052]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-72052]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-72052] - 3.75ug/ml staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NDRG2 Antibody [NBP1-72052]

Western Blot: NDRG2 Antibody [NBP1-72052]

Western Blot: NDRG2 Antibody [NBP1-72052] - Staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for NDRG2 Antibody

Application
Recommended Usage

Immunohistochemistry

0.1-0.3ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1:1000
Application Notes
Western blot: Approx 45kDa band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kDa according to NP_963293.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Adrenal Gland.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDRG2

NDRG2 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

DKFZp781G1938, FLJ25522, KIAA1248cytoplasmic protein Ndr1, NDR1-related protein NDR2, NDRG family member 2, protein NDRG2, Protein Syld709613, syld709613 protein, SYLDN-myc downstream regulator 2

Entrez Gene IDs

57447 (Human); 29811 (Mouse); 171114 (Rat)

Gene Symbol

NDRG2

UniProt

NP_963293.1 (Human)

Additional NDRG2 Products

Product Documents for NDRG2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDRG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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