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NDUFA7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31758

Novus Biologicals, part of Bio-Techne
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NBP2-31758
NBP2-31758-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SATRLIQRLRNWASGHDLQGKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFA7 Antibody

Western Blot: NDUFA7 Antibody [NBP2-31758]

Western Blot: NDUFA7 Antibody [NBP2-31758]

Western Blot: NDUFA7 Antibody [NBP2-31758] - Analysis in human cell line HEK 293 and human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: NDUFA7 Antibody [NBP2-31758]

Immunocytochemistry/ Immunofluorescence: NDUFA7 Antibody [NBP2-31758]

Immunocytochemistry/Immunofluorescence: NDUFA7 Antibody [NBP2-31758] - Staining of human cell line PC-3 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-31758]

Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-31758]

Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-31758] - Staining of human pancreas shows weak positivity in exocrine glandular cells.

Applications for NDUFA7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFA7

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Alternate Names

B14.5a, CI-B14.5a, complex I B14.5a subunit, Complex I-B14.5a, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5kD, B14.5a), NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NADH-ubiquinone oxidoreductase subunit B14.5a

Gene Symbol

NDUFA7

UniProt

Additional NDUFA7 Products

Product Documents for NDUFA7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFA7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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