NDUFAF1 Antibody (4K8Y8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16313
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4K8Y8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFAF1 (Q9Y375). MALVHKLLRGTYFLRKFSKPTSALYPFLGIRFAEYSSSLQKPVASPGKASSQRKTEGDLQGDHQKEVALDITSSEEKPDVSFDKAIRDEAIYHFRLLKDEIVDHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NDUFAF1 Antibody (4K8Y8)
![Western Blot: NDUFAF1 Antibody (4K8Y8) [NBP3-16313]](https://resources.bio-techne.com/images/products/NDUFAF1-Antibody-4K8Y8-Western-Blot-NBP3-16313-img0002.jpg "Western Blot: NDUFAF1 Antibody (4K8Y8) [NBP3-16313]")
Western Blot: NDUFAF1 Antibody (4K8Y8) [NBP3-16313]
Western Blot: NDUFAF1 Antibody (4K8Y8) [NBP3-16313] - Western blot analysis of extracts of various cell lines, using NDUFAF1 Rabbit mAb (NBP3-16313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody (4K8Y8) [NBP3-16313]](https://resources.bio-techne.com/images/products/NDUFAF1-Antibody-4K8Y8-Immunocytochemistry-Immunofluorescence-NBP3-16313-img0001.jpg "Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody (4K8Y8) [NBP3-16313]")
Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody (4K8Y8) [NBP3-16313]
Immunocytochemistry/Immunofluorescence: NDUFAF1 Antibody (4K8Y8) [NBP3-16313] - Immunofluorescence analysis of HeLa cells using NDUFAF1 Rabbit mAb (NBP3-16313) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for NDUFAF1 Antibody (4K8Y8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NDUFAF1
Alternate Names
CGI-65, CIA30CGI65, complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1
Gene Symbol
NDUFAF1
Additional NDUFAF1 Products
Product Documents for NDUFAF1 Antibody (4K8Y8)
Product Specific Notices for NDUFAF1 Antibody (4K8Y8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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