NDUFAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84503

Novus Biologicals, part of Bio-Techne
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NBP1-84503
NBP1-84503-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDUFAF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLKDEIVDHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEVFLKMGKNNQSALLYGTLSSEAPQDGESTRSGYCAMISRIPRGAF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFAF1 Antibody

Western Blot: NDUFAF1 Antibody [NBP1-84503]

Western Blot: NDUFAF1 Antibody [NBP1-84503]

Western Blot: NDUFAF1 Antibody [NBP1-84503] - Analysis in control (vector only transfected HEK293T lysate) and NDUFAF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody [NBP1-84503]

Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody [NBP1-84503]

Immunocytochemistry/Immunofluorescence: NDUFAF1 Antibody [NBP1-84503] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: NDUFAF1 Antibody [NBP1-84503]

Immunohistochemistry-Paraffin: NDUFAF1 Antibody [NBP1-84503]

Immunohistochemistry-Paraffin: NDUFAF1 Antibody [NBP1-84503] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for NDUFAF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFAF1

The NDUFAF1 gene encodes the human homolog of a Neurospora crassa Cia30 protein involved in the assembly of complex I.[supplied by OMIM]

Alternate Names

CGI-65, CIA30CGI65, complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1

Gene Symbol

NDUFAF1

Additional NDUFAF1 Products

Product Documents for NDUFAF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDUFAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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