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NDUFAF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92171

Novus Biologicals, part of Bio-Techne
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NBP1-92171
NBP1-92171-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IKSIPKGKISIVEALTLLNNHKLFPETWTAEKIMQEYQLEQKDVNSLLKYFVTFEVEIFPPEDKKAIRSK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFAF4 Antibody

Immunocytochemistry/ Immunofluorescence: NDUFAF4 Antibody [NBP1-92171]

Immunocytochemistry/ Immunofluorescence: NDUFAF4 Antibody [NBP1-92171]

Immunocytochemistry/Immunofluorescence: NDUFAF4 Antibody [NBP1-92171] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: NDUFAF4 Antibody [NBP1-92171]

Immunohistochemistry-Paraffin: NDUFAF4 Antibody [NBP1-92171]

Immunohistochemistry-Paraffin: NDUFAF4 Antibody [NBP1-92171] - Staining of human prostate shows moderate membranous and cytoplasmic positivity in glandular cells.

Applications for NDUFAF4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFAF4

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency. (provided by RefSeq)

Alternate Names

bA22L21.1, C6orf66chromosome 6 open reading frame 66, Hormone-regulated proliferation-associated protein of 20 kDa, hormone-regulated proliferation-associated protein, 20 kDa, HRPAP20HSPC125, My013, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4

Gene Symbol

NDUFAF4

Additional NDUFAF4 Products

Product Documents for NDUFAF4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFAF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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