NDUFB10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19503

Novus Biologicals, part of Bio-Techne
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NBP2-19503
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDUFB10 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFB10. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (98%), Rhesus Monkey (83%).

Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFB10 Antibody

Immunocytochemistry/ Immunofluorescence: NDUFB10 Antibody [NBP2-19503]

Immunocytochemistry/ Immunofluorescence: NDUFB10 Antibody [NBP2-19503]

Immunocytochemistry/Immunofluorescence: NDUFB10 Antibody [NBP2-19503] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NDUFB10 Antibody [NBP2-19503]

Immunohistochemistry-Paraffin: NDUFB10 Antibody [NBP2-19503]

Immunohistochemistry-Paraffin: NDUFB10 Antibody [NBP2-19503] - Human breast cancer, using NDUFB10 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
NDUFB10 Antibody

Western Blot: NDUFB10 Antibody [NBP2-19503] -

Western Blot: NDUFB10 Antibody [NBP2-19503] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (NBP2-19503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Applications for NDUFB10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFB10

NDUFB10 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Complex I is composed of 45 different subunits.

Alternate Names

CI-PDSW, complex I PDSW subunit, Complex I-PDSW, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (22kD, PDSW), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3), NADH-ubiquinone oxidoreductase PDSW subunit, PDSW

Gene Symbol

NDUFB10

UniProt

O96000 (Human)

Additional NDUFB10 Products

Product Documents for NDUFB10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NDUFB10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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