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NDUFB10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38064

Novus Biologicals, part of Bio-Techne
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NBP3-38064-100ul
NBP3-38064-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1).

Sequence:
MPDSWDKDVYPEPPRRTPVQPNPIVYMMKAFDLIVDRPVTLVREFIERQHAKNRYYYYHRQYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKACQQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQERKAAKEAAAATS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFB10 Antibody

NDUFB10 Antibody

Immunoprecipitation: NDUFB10 Antibody [NBP3-38064] -

Immunoprecipitation: NDUFB10 Antibody [NBP3-38064] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug NDUFB10 antibody. Western blot was performed from the immunoprecipitate using NDUFB10 antibody at a dilution of 1:1000.
NDUFB10 Antibody

Western Blot: NDUFB10 Antibody [NBP3-38064] -

Western Blot: NDUFB10 Antibody [NBP3-38064] - Western blot analysis of various lysates using NDUFB10 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for NDUFB10 Antibody

Application
Recommended Usage

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFB10

NDUFB10 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Complex I is composed of 45 different subunits.

Alternate Names

CI-PDSW, complex I PDSW subunit, Complex I-PDSW, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (22kD, PDSW), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3), NADH-ubiquinone oxidoreductase PDSW subunit, PDSW

Gene Symbol

NDUFB10

Additional NDUFB10 Products

Product Documents for NDUFB10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFB10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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