NDUFB4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33626

Novus Biologicals, part of Bio-Techne
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NBP2-33626
NBP2-33626-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDUFB4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFB4 Antibody

Western Blot: NDUFB4 Antibody [NBP2-33626]

Western Blot: NDUFB4 Antibody [NBP2-33626]

Western Blot: NDUFB4 Antibody [NBP2-33626] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: NDUFB4 Antibody [NBP2-33626]

Immunocytochemistry/ Immunofluorescence: NDUFB4 Antibody [NBP2-33626]

Immunocytochemistry/Immunofluorescence: NDUFB4 Antibody [NBP2-33626] - Staining of human cell line U-2 OS shows localization to nuclear membrane & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-33626]

Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-33626]

Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-33626] - Staining of human pancreas shows low expression as expected.
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Applications for NDUFB4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFB4

The NDUFB4 gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electr

Alternate Names

B15, CI-B15, complex I B15 subunit, Complex I-B15, MGC5105, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4 (15kD, B15), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NADH-ubiquinone oxidoreductase B15 subunit

Gene Symbol

NDUFB4

UniProt

O95168 (Human)

Additional NDUFB4 Products

Product Documents for NDUFB4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NDUFB4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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