NDUFB6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12619

Novus Biologicals, part of Bio-Techne
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NBP3-12619-100ul
NBP3-12619-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NDUFB6 Antibodies »

Product Summary for NDUFB6 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human NDUFB6 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for NDUFB6 Antibody

Western Blot: NDUFB6 Antibody [NBP3-12619]

Western Blot: NDUFB6 Antibody [NBP3-12619]

Western Blot: NDUFB6 Antibody [NBP3-12619] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:18 kDa
Immunocytochemistry/ Immunofluorescence: NDUFB6 Antibody [NBP3-12619]

Immunocytochemistry/ Immunofluorescence: NDUFB6 Antibody [NBP3-12619]

Immunocytochemistry/Immunofluorescence: NDUFB6 Antibody [NBP3-12619] - Staining of NDUFB6 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NDUFB6 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation: NDUFB6 Antibody [NBP3-12619]

Immunoprecipitation: NDUFB6 Antibody [NBP3-12619]

Immunoprecipitation: NDUFB6 Antibody [NBP3-12619] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-NDUFB6 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NDUFB6 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :20 kDa

Applications for NDUFB6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFB6

NDUFB6 is encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Alternate Names

B17, CI, CI-B17, complex I, mitochondrial respiratory chain, B17 subunit, Complex I-B17, MGC13675, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6 (17kD, B17), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6, NADH-ubiquinone oxidoreductase B17 subunit, NADH-ubiquinone oxidoreductase beta subunit, 6

Gene Symbol

NDUFB6

Additional NDUFB6 Products

Product Documents for NDUFB6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFB6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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