NDUFB8 Antibody (6T8G1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15882

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15882-100ul
NBP3-15882-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6T8G1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NDUFB8 Antibodies »

Product Summary for NDUFB8 Antibody (6T8G1)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFB8 (O95169). MAVARAGVLGVQWLQRASRNVMPLGARTASHMTKDMFPGPYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPDRSQHERDPWYSWDQPGLRLNWGEPMHWHLDMYNRNRVDTSPTPVSWHVMCMQLFGFLAFMIFMCWVGDVY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFB8 Antibody (6T8G1)

Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]

Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]

Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882] - Western blot analysis of extracts of various cell lines, using NDUFB8 Rabbit mAb (NBP3-15882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: NDUFB8 Antibody (6T8G1) [NBP3-15882]

Immunocytochemistry/ Immunofluorescence: NDUFB8 Antibody (6T8G1) [NBP3-15882]

Immunocytochemistry/Immunofluorescence: NDUFB8 Antibody (6T8G1) [NBP3-15882] - Immunofluorescence analysis of U-2 OS cells using NDUFB8 Rabbit mAb (NBP3-15882) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: NDUFB8 Antibody (6T8G1) [NBP3-15882]

Immunohistochemistry-Paraffin: NDUFB8 Antibody (6T8G1) [NBP3-15882]

Immunohistochemistry-Paraffin: NDUFB8 Antibody (6T8G1) [NBP3-15882] - Immunohistochemistry of paraffin-embedded mouse kidney using NDUFB8 Rabbit mAb (NBP3-15882) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for NDUFB8 Antibody (6T8G1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFB8

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Alternate Names

CI-ASHImitochondrial, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (19kD, ASHI), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa, NADH:ubiquinone oxidoreductase ASHI subunit, NADH-ubiquinone oxidoreductase ASHI subunit

Gene Symbol

NDUFB8

Additional NDUFB8 Products

Product Documents for NDUFB8 Antibody (6T8G1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDUFB8 Antibody (6T8G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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