NDUFB8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05965

Novus Biologicals, part of Bio-Techne
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NBP3-05965-100ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NDUFB8 Antibodies »

Product Summary for NDUFB8 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human NDUFB8 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for NDUFB8 Antibody

Western Blot: NDUFB8 Antibody [NBP3-05965]

Western Blot: NDUFB8 Antibody [NBP3-05965]

Western Blot: NDUFB8 Antibody [NBP3-05965] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa
Immunocytochemistry/ Immunofluorescence: NDUFB8 Antibody [NBP3-05965]

Immunocytochemistry/ Immunofluorescence: NDUFB8 Antibody [NBP3-05965]

Immunocytochemistry/Immunofluorescence: NDUFB8 Antibody [NBP3-05965] - Staining of NDUFB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NDUFB8 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: NDUFB8 Antibody [NBP3-05965]

Immunohistochemistry-Paraffin: NDUFB8 Antibody [NBP3-05965]

Immunohistochemistry-Paraffin: NDUFB8 Antibody [NBP3-05965] - Staining of human NDUFB8 in human kidney with rabbit polyclonal antibody at 1:100 dilution.
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Applications for NDUFB8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFB8

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Alternate Names

CI-ASHImitochondrial, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (19kD, ASHI), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa, NADH:ubiquinone oxidoreductase ASHI subunit, NADH-ubiquinone oxidoreductase ASHI subunit

Gene Symbol

NDUFB8

Additional NDUFB8 Products

Product Documents for NDUFB8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFB8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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