NDUFB9 Antibody (OTI8B7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72918
![Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918]](https://resources.bio-techne.com/images/products/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72918-img0003.jpg "Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918]")
![Western Blot: NDUFB9 Antibody (OTI8B7)Azide and BSA Free [NBP2-72918]](https://resources.bio-techne.com/images/products/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Western-Blot-NBP2-72918-img0002.jpg "Western Blot: NDUFB9 Antibody (OTI8B7)Azide and BSA Free [NBP2-72918]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey, Primate
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI8B7
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human fragment corresponding to amino acids 3-179 of human NDUFB9 (NP_001893) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
21.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NDUFB9 Antibody (OTI8B7) - Azide and BSA Free
Western Blot: NDUFB9 Antibody (OTI8B7)Azide and BSA Free [NBP2-72918]
Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB9.![Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918]](https://resources.bio-techne.com/images/products/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72918-img0004.jpg "Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918]")
Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918]
Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of Jurkat cells, using anti-NDUFB9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).![Western Blot: NDUFB9 Antibody (OTI8B7)Azide and BSA Free [NBP2-72918]](https://resources.bio-techne.com/images/products/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Western-Blot-NBP2-72918-img0001.jpg "Western Blot: NDUFB9 Antibody (OTI8B7)Azide and BSA Free [NBP2-72918]")
Western Blot: NDUFB9 Antibody (OTI8B7)Azide and BSA Free [NBP2-72918]
Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NDUFB9 monoclonal antibody.Applications for NDUFB9 Antibody (OTI8B7) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Western Blot
1:1000-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NDUFB9
Alternate Names
B22, CI-B22, complex I B22 subunit, Complex I-B22, FLJ22885, LYR motif-containing protein 3, LYRM3DKFZp566O173, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, NADH-ubiquinone oxidoreductase B22 subunit, UQOR22NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (22kD, B22)
Gene Symbol
NDUFB9
Additional NDUFB9 Products
Product Documents for NDUFB9 Antibody (OTI8B7) - Azide and BSA Free
Product Specific Notices for NDUFB9 Antibody (OTI8B7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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