NDUFS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30413

Novus Biologicals, part of Bio-Techne
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NBP2-30413
NBP2-30413-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDUFS2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFS2 Antibody

Immunohistochemistry-Paraffin: NDUFS2 Antibody [NBP2-30413]

Immunohistochemistry-Paraffin: NDUFS2 Antibody [NBP2-30413]

Immunohistochemistry-Paraffin: NDUFS2 Antibody [NBP2-30413] - Staining in human heart muscle and pancreas tissues using anti-NDUFS2 antibody. Corresponding NDUFS2 RNA-seq data are presented for the same tissues.
Western Blot: NDUFS2 Antibody [NBP2-30413]

Western Blot: NDUFS2 Antibody [NBP2-30413]

Western Blot: NDUFS2 Antibody [NBP2-30413] - Analysis using Anti-NDUFS2 antibody NBP2-30413 (A) shows similar pattern to independent antibody NBP2-49351 (B).
Immunocytochemistry/ Immunofluorescence: NDUFS2 Antibody [NBP2-30413]

Immunocytochemistry/ Immunofluorescence: NDUFS2 Antibody [NBP2-30413]

Immunocytochemistry/Immunofluorescence: NDUFS2 Antibody [NBP2-30413] - Staining of human cell line HeLa shows localization to mitochondria. Antibody staining is shown in green.
View 2 more images

Applications for NDUFS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFS2

Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM]

Alternate Names

CI-49, CI-49kD, complex 1, mitochondrial respiratory chain, 49-KD subunit, complex I 49kDa subunit, Complex I-49kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 2 (49kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, NADH-ubiquinone oxidoreductase 49 kDa subunit, NADH-ubiquinone oxidoreductase NDUFS2 subunit

Gene Symbol

NDUFS2

UniProt

O75306 (Human)

Additional NDUFS2 Products

Product Documents for NDUFS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDUFS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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