NDUFS3 Antibody (1D6)

Novus Biologicals, part of Bio-Techne | Catalog # H00004722-M02

Novus Biologicals, part of Bio-Techne
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H00004722-M02-100ug
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1D6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDUFS3 Antibodies »

Product Specifications

Immunogen

NDUFS3 (AAH00617, 1 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAAVARLWWRGILGASALTRGTGRPSVLLLPVRRESAGADTRPTVRPRNDVAHKQLSAFGEYVAEILPKYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLTAVDVPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESAVSVFKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK

Specificity

NDUFS3 - NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NDUFS3 Antibody (1D6)

Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02]

Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02]

Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 expression in transfected 293T cell line by NDUFS3 monoclonal antibody (M02), clone 1D6.Lane 1: NDUFS3 transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: NDUFS3 Antibody (1D6) [H00004722-M02]

Immunohistochemistry-Paraffin: NDUFS3 Antibody (1D6) [H00004722-M02]

Immunohistochemistry-Paraffin: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of monoclonal antibody to NDUFS3 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02]

Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02]

Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 over-expressed 293 cell line, cotransfected with NDUFS3 Validated Chimera RNAi ( Cat # H00004722-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS3 monoclonal antibody (M02), clone 1D6 (Cat # H00004722-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for NDUFS3 Antibody (1D6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFS3

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. See NDUFS1 (MIM 157655).[supplied by OMIM]

Alternate Names

CI-30, CI-30kD, complex I 30kDa subunit, Complex I-30kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit

Entrez Gene IDs

4722 (Human)

Gene Symbol

NDUFS3

OMIM

256000 (Human)

UniProt

AAH00617 (Human)

Additional NDUFS3 Products

Product Documents for NDUFS3 Antibody (1D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDUFS3 Antibody (1D6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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