NDUFS3 Antibody (1H12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26117
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1H12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human NDUFS3 [UniProt O75489]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NDUFS3 Antibody (1H12)
Immunohistochemistry: NDUFS3 Antibody (1H12) [NBP3-26117] -
Immunohistochemistry: NDUFS3 Antibody (1H12) [NBP3-26117] - Image of NDUFS3 Antibody (1H12) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: NDUFS3 Antibody (1H12) [NBP3-26117] -
Western Blot: NDUFS3 Antibody (1H12) [NBP3-26117] - Positive Western Blot detected in: 293 whole cell lysate, HepG2 whole cell lysate.All lanes: NDUFS3 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 31, 15 KDa
Observed band size: 31 kDa
Applications for NDUFS3 Antibody (1H12)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: NDUFS3
Alternate Names
CI-30, CI-30kD, complex I 30kDa subunit, Complex I-30kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit
Gene Symbol
NDUFS3
Additional NDUFS3 Products
Product Documents for NDUFS3 Antibody (1H12)
Product Specific Notices for NDUFS3 Antibody (1H12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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