NDUFS3 Antibody (JU10-32)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75587

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU10-32

Concentration

1 mg/ml

View all NDUFS3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human NDUFS3 aa 215-264 / 264. (SwissProt: O75489 Human)

Localization

Mitochondrion inner membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFS3 Antibody (JU10-32)

Western Blot: NDUFS3 Antibody (JU10-32) [NBP2-75587]

Western Blot: NDUFS3 Antibody (JU10-32) [NBP2-75587]

Western Blot: NDUFS3 Antibody (JU10-32) [NBP2-75587] - Analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: NDUFS3 Antibody (JU10-32) [NBP2-75587]

Immunocytochemistry/ Immunofluorescence: NDUFS3 Antibody (JU10-32) [NBP2-75587]

Immunocytochemistry/Immunofluorescence: NDUFS3 Antibody (JU10-32) [NBP2-75587] - Staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: NDUFS3 Antibody (JU10-32) [NBP2-75587]

Immunohistochemistry-Paraffin: NDUFS3 Antibody (JU10-32) [NBP2-75587]

Immunohistochemistry-Paraffin: NDUFS3 Antibody (JU10-32) [NBP2-75587] - Analysis of paraffin-embedded human kidney cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.
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Applications for NDUFS3 Antibody (JU10-32)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

1:10-1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFS3

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. See NDUFS1 (MIM 157655).[supplied by OMIM]

Alternate Names

CI-30, CI-30kD, complex I 30kDa subunit, Complex I-30kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit

Gene Symbol

NDUFS3

Additional NDUFS3 Products

Product Documents for NDUFS3 Antibody (JU10-32)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDUFS3 Antibody (JU10-32)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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