NDUFS3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19505

Novus Biologicals, part of Bio-Techne
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NBP2-19505
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (94%), Chicken (90%), Mouse (92%), Porcine (93%), Primate (96%), Rat (91%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDUFS3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS3. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (96%), Zebrafish (81%), Xenopus laevis (83%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFS3 Antibody

Knockout Validated: NDUFS3 Antibody [NBP2-19505]

Knockout Validated: NDUFS3 Antibody [NBP2-19505]

Knockout Validated: NDUFS3 Antibody [NBP2-19505] - Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: NDUFS3 Antibody [NBP2-19505]

Western Blot: NDUFS3 Antibody [NBP2-19505]

Western Blot: NDUFS3 Antibody [NBP2-19505] - . Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: NDUFS3 Antibody [NBP2-19505]

Immunohistochemistry-Paraffin: NDUFS3 Antibody [NBP2-19505]

Immunohistochemistry-Paraffin: NDUFS3 Antibody [NBP2-19505] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
View 1 more images

Applications for NDUFS3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFS3

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Alternate Names

CI-30, CI-30kD, complex I 30kDa subunit, Complex I-30kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit

Gene Symbol

NDUFS3

UniProt

O75489 (Human)

Additional NDUFS3 Products

Product Documents for NDUFS3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NDUFS3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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