Nebulin Antibody (NB2)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-11099
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Alligator, Amphibian, Avian, Bovine, Chicken, Fish, Rabbit
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # NB2
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Full length native protein (purified) (Chicken).
Reactivity Notes
Cross-reacts with a wide range of species, including salamander, pigeon, alligator, toad. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cytoplasmic
Specificity
Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Nebulin Antibody (NB2)
![Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099]](https://resources.bio-techne.com/images/products/Nebulin-Antibody-NB2-Immunohistochemistry-NB120-11099-img0001.jpg "Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099]")
Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099]
Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099] - Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:400 Monoclonal Anti-Nebulin antibody produced in Mouse, Clone: NB2. followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.Applications for Nebulin Antibody (NB2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:400
Western Blot
1:100-1:2000
Application Notes
IF: Use at a dilution of 1/400. WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. By indirect immunofluorescence, the antibody shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22 um from the z-line in electron micrographs. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.9% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nebulin
Alternate Names
DKFZp686C1456, FLJ11505, FLJ36536, FLJ39568, FLJ39584, NEB177D, nebulin, NEM2
Gene Symbol
NEB
Additional Nebulin Products
Product Documents for Nebulin Antibody (NB2)
Product Specific Notices for Nebulin Antibody (NB2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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