Necdin Antibody (OTI5D1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72925

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5D1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Necdin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NDN(NP_002478) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Necdin Antibody (OTI5D1) - Azide and BSA Free

Western Blot: Necdin Antibody (OTI5D1)Azide and BSA Free [NBP2-72925]

Western Blot: Necdin Antibody (OTI5D1)Azide and BSA Free [NBP2-72925]

Western Blot: Necdin Antibody (OTI5D1) - Azide and BSA Free [NBP2-72925] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Necdin.
Immunocytochemistry/ Immunofluorescence: Necdin Antibody (OTI5D1) - Azide and BSA Free [NBP2-72925]

Immunocytochemistry/ Immunofluorescence: Necdin Antibody (OTI5D1) - Azide and BSA Free [NBP2-72925]

Immunocytochemistry/Immunofluorescence: Necdin Antibody (OTI5D1) - Azide and BSA Free [NBP2-72925] - Analysis of HeLa cells.

Applications for Necdin Antibody (OTI5D1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Necdin

Necdin is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.

Alternate Names

HsT16328, necdin, necdin (mouse) homolog, necdin homolog (mouse), PWCR

Gene Symbol

NDN

Additional Necdin Products

Product Documents for Necdin Antibody (OTI5D1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Necdin Antibody (OTI5D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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