NEDD4 Antibody (JE35-58)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32641
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE35-58
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment corresponding to Human NEDD4 aa 160-460. (Uniprot: P46934)
Localization
Cytoplasm, Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
149 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NEDD4 Antibody (JE35-58)
Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641]
Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution.Lane 1: U87MG cell lysate
Lane 2: A549 cell lysate
Lane 3: NIH/3T3 cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 149 kDa
Observed band size: 110 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Applications for NEDD4 Antibody (JE35-58)
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NEDD4
Long Name
Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4
Alternate Names
RPF1
Gene Symbol
NEDD4
Additional NEDD4 Products
Product Documents for NEDD4 Antibody (JE35-58)
Product Specific Notices for NEDD4 Antibody (JE35-58)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...