NEDD4L Antibody (1D2)
Novus Biologicals, part of Bio-Techne | Catalog # H00023327-M04
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1D2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NEDD4L (AAH32597, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLT
Reactivity Notes
Human. Other species not tested.
Specificity
NEDD4L (1D2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for NEDD4L Antibody (1D2)
![Western Blot: NEDD4L Antibody (1D2) [H00023327-M04]](https://resources.bio-techne.com/images/products/NEDD4L-Antibody-1D2-Western-Blot-H00023327-M04-img0003.jpg "Western Blot: NEDD4L Antibody (1D2) [H00023327-M04]")
Western Blot: NEDD4L Antibody (1D2) [H00023327-M04]
Western Blot: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2. Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa). Lane 2: Non-transfected lysate.![Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04]](https://resources.bio-techne.com/images/products/NEDD4L-Antibody-1D2-Immunoprecipitation-H00023327-M04-img0001.jpg "Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04]")
Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04]
Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEDD4L monoclonal antibody.
Sandwich ELISA: NEDD4L Antibody (1D2) [H00023327-M04] - Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody.
Applications for NEDD4L Antibody (1D2)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NEDD4L
Alternate Names
E3 ubiquitin-protein ligase NEDD4-like, EC 6.3.2, EC 6.3.2.-, FLJ33870, hNedd4-2, KIAA0439ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDD4-2, NEDL3, neural precursor cell expressed, developmentally down-regulated 4-like, neural precursor cell expressed, developmentally down-regulated gene 4-like, RSP5ubiquitin-protein ligase Rsp5
Entrez Gene IDs
23327 (Human)
Gene Symbol
NEDD4L
OMIM
606384 (Human)
UniProt
Additional NEDD4L Products
Product Documents for NEDD4L Antibody (1D2)
Product Specific Notices for NEDD4L Antibody (1D2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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