NEDD4L Antibody (3W9J2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16747

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16747-100ul
NBP3-16747-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3W9J2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NEDD4L Antibodies »

Product Summary for NEDD4L Antibody (3W9J2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 876-975 of human NEDD4L (Q96PU5). NHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEDD4L Antibody (3W9J2)

Western Blot: NEDD4L Antibody (3W9J2) [NBP3-16747]

Western Blot: NEDD4L Antibody (3W9J2) [NBP3-16747]

Western Blot: NEDD4L Antibody (3W9J2) [NBP3-16747] - Western blot analysis of extracts of various cell lines, using NEDD4L antibody (NBP3-16747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for NEDD4L Antibody (3W9J2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEDD4L

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK

Alternate Names

E3 ubiquitin-protein ligase NEDD4-like, EC 6.3.2, EC 6.3.2.-, FLJ33870, hNedd4-2, KIAA0439ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDD4-2, NEDL3, neural precursor cell expressed, developmentally down-regulated 4-like, neural precursor cell expressed, developmentally down-regulated gene 4-like, RSP5ubiquitin-protein ligase Rsp5

Gene Symbol

NEDD4L

Additional NEDD4L Products

Product Documents for NEDD4L Antibody (3W9J2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NEDD4L Antibody (3W9J2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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