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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 186-390 of human NEIL1 (NP_078884) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEIL1 Antibody (OTI1D1) - Azide and BSA Free

Western Blot: NEIL1 Antibody (OTI1D1)Azide and BSA Free [NBP2-72932]

Western Blot: NEIL1 Antibody (OTI1D1)Azide and BSA Free [NBP2-72932]

Western Blot: NEIL1 Antibody (OTI1D1) - Azide and BSA Free [NBP2-72932] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEIL1.

Applications for NEIL1 Antibody (OTI1D1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:200

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEIL1

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002).[supplied by OMIM]

Alternate Names

DNA-(apurinic or apyrimidinic site) lyase Neil1, EC 3.2.2.-, EC 4.2.99.18, endonuclease 8-like 1, endonuclease VIII, Endonuclease VIII-like 1, FLJ22402, FPG1DNA glycosylase/AP lyase Neil1, hFPG1, NEH1, nei endonuclease VIII-like 1 (E. coli), Nei homolog 1, NEI1, Nei-like protein 1

Gene Symbol

NEIL1

Additional NEIL1 Products

Product Documents for NEIL1 Antibody (OTI1D1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NEIL1 Antibody (OTI1D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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