NEIL2 Antibody (1B7)
Novus Biologicals, part of Bio-Techne | Catalog # H00252969-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1B7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NEIL2 (AAH13964, 1 a.a. ~ 332 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPEGPLVRKFHHLVSPFVGQQVVKTGGSSKKLQPASLQSLWLQDTQVHGKKLFLRFDLDEEMGPPGSSPTPEPPQKEVQKEGAADPKQVGEPSGQKTLDGSSRSAELVPQGEDDSEYLERDAPAGDAGRWLRVSFGLFGSVWVNDFSRAKKANKRGDWRDPSPRLVLHFGGGGFLAFYNCQLSWSSSPVVTPTCDILSEKFHRGQALEALGQAQPVCYTLLDQRYFSGLGNIIKNEALYRAGIHPLSLGSVLSASRREVLVDHVVEFSTAWLQGKFQGRPQHTQVYQKEQCPAGHQVMKEAFGPEDGLQRLTWWCPQCQPQLSEEPEQCQFS
Specificity
NEIL2 - nei like 2 (E. coli)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for NEIL2 Antibody (1B7)
![Western Blot: NEIL2 Antibody (1B7) [H00252969-M01]](https://resources.bio-techne.com/images/products/NEIL2-Antibody-1B7-Western-Blot-H00252969-M01-img0006.jpg "Western Blot: NEIL2 Antibody (1B7) [H00252969-M01]")
Western Blot: NEIL2 Antibody (1B7) [H00252969-M01]
Western Blot: NEIL2 Antibody (1B7) [H00252969-M01] - Analysis of NEIL2 expression in transfected 293T cell line by NEIL2 monoclonal antibody (M01), clone 1B7.Lane 1: NEIL2 transfected lysate(36.8 KDa).Lane 2: Non-transfected lysate.![Western Blot: NEIL2 Antibody (1B7) [H00252969-M01]](https://resources.bio-techne.com/images/products/NEIL2-Antibody-1B7-Western-Blot-H00252969-M01-img0004.jpg "Western Blot: NEIL2 Antibody (1B7) [H00252969-M01]")
Western Blot: NEIL2 Antibody (1B7) [H00252969-M01]
Western Blot: NEIL2 Antibody (1B7) [H00252969-M01] - Analysis of NEIL2 over-expressed 293 cell line, cotransfected with NEIL2 Validated Chimera RNAi ( Cat # H00252969-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NEIL2 monoclonal antibody (M01), clone 1B7 (Cat # H00252969-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![ELISA: NEIL2 Antibody (1B7) [H00252969-M01]](https://resources.bio-techne.com/images/products/NEIL2-Antibody-1B7-ELISA-H00252969-M01-img0007.jpg "ELISA: NEIL2 Antibody (1B7) [H00252969-M01]")
ELISA: NEIL2 Antibody (1B7) [H00252969-M01]
ELISA: NEIL2 Antibody (1B7) [H00252969-M01] - Detection limit for recombinant GST tagged NEIL2 is approximately 1ng/ml as a capture antibody.Applications for NEIL2 Antibody (1B7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NEIL2
Alternate Names
DNA glycosylase/AP lyase Neil2, EC 3.2.2.-, EC 4.2.99.18, endonuclease 8-like 2, Endonuclease VIII-like 2, FLJ31644, MGC2832, MGC4505, NEH2DNA-(apurinic or apyrimidinic site) lyase Neil2, nei endonuclease VIII-like 2 (E. coli), Nei homolog 2, nei like 2, nei like 2 (E. coli), NEI2, Nei-like protein 2
Entrez Gene IDs
252969 (Human)
Gene Symbol
NEIL2
OMIM
608933 (Human)
UniProt
Additional NEIL2 Products
Product Documents for NEIL2 Antibody (1B7)
Product Specific Notices for NEIL2 Antibody (1B7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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