NEK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36534
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation (Negative), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 1208 to 1258 of human Never in Mitosis A-Related Kinase 1 using the numbering given in entry NP_036356.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NEK1 Antibody
Western Blot: NEK1 Antibody [NBP2-36534]
Western Blot: NEK1 Antibody [NBP2-36534] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiNEK1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Western Blot: NEK1 Antibody [NBP2-36534]
Western Blot: NEK1 Antibody [NBP2-36534] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEK1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for NEK1 Antibody
Application
Recommended Usage
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NEK1
Alternate Names
DKFZp686D06121, DKFZp686K12169, EC 2.7.11, EC 2.7.11.1, KIAA1901MGC138800, Never in mitosis A-related kinase 1, NIMA (never in mitosis gene a)-related kinase 1, NimA-related protein kinase 1, NY-REN-55, protein-serine/threonine kinase, Renal carcinoma antigen NY-REN-55, serine/threonine-protein kinase Nek1, SRPS2
Gene Symbol
NEK1
UniProt
Additional NEK1 Products
Product Documents for NEK1 Antibody
Product Specific Notices for NEK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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