Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NEK11 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NEK11(NP_079076) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK11 Antibody (OTI5D9) - Azide and BSA Free

Western Blot: NEK11 Antibody (OTI5D9)Azide and BSA Free [NBP2-72935]

Western Blot: NEK11 Antibody (OTI5D9)Azide and BSA Free [NBP2-72935]

Western Blot: NEK11 Antibody (OTI5D9) - Azide and BSA Free [NBP2-72935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11.
Immunocytochemistry/ Immunofluorescence: NEK11 Antibody (OTI5D9) - Azide and BSA Free [NBP2-72935]

Immunocytochemistry/ Immunofluorescence: NEK11 Antibody (OTI5D9) - Azide and BSA Free [NBP2-72935]

Immunocytochemistry/Immunofluorescence: NEK11 Antibody (OTI5D9) - Azide and BSA Free [NBP2-72935] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY NEK11.

Applications for NEK11 Antibody (OTI5D9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK11

NEK11 belongs to the NIMA family of kinases, which are involved in DNA replication and genotoxic stress responses (Noguchi et al., 2002 [PubMed 12154088]).[supplied by OMIM]

Alternate Names

EC 2.7.11, EC 2.7.11.1, FLJ23495, Never in mitosis A-related kinase 11, NIMA (never in mitosis gene a)- related kinase 11, NimA-related protein kinase 11, serine/threonine-protein kinase Nek11

Gene Symbol

NEK11

Additional NEK11 Products

Product Documents for NEK11 Antibody (OTI5D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NEK11 Antibody (OTI5D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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