NEK3 Antibody (2F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00004752-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEK3 Antibodies »

Product Specifications

Immunogen

NEK3 (AAH19916, 406 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QWLKETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEETEASDSVDGGHDSVILDPERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR

Specificity

NEK3 - NIMA (never in mitosis gene a)-related kinase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NEK3 Antibody (2F8)

Western Blot: NEK3 Antibody (2F8) [H00004752-M01]

Western Blot: NEK3 Antibody (2F8) [H00004752-M01]

Western Blot: NEK3 Antibody (2F8) [H00004752-M01] - Analysis of NEK3 expression in transfected 293T cell line by NEK3 monoclonal antibody (M01), clone 2F8.Lane 1: NEK3 transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NEK3 Antibody (2F8) [H00004752-M01]

Immunocytochemistry/ Immunofluorescence: NEK3 Antibody (2F8) [H00004752-M01]

Immunocytochemistry/Immunofluorescence: NEK3 Antibody (2F8) [H00004752-M01] - Analysis of monoclonal antibody to NEK3 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: NEK3 Antibody (2F8) [H00004752-M01]

ELISA: NEK3 Antibody (2F8) [H00004752-M01]

ELISA: NEK3 Antibody (2F8) [H00004752-M01] - Detection limit for recombinant GST tagged NEK3 is approximately 0.03ng/ml as a capture antibody.

Applications for NEK3 Antibody (2F8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NEK3

In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.

Alternate Names

EC 2.7.11, EC 2.7.11.1, glycogen synthase A kinase, HSPK 36, HSPK36, hydroxyalkyl-protein kinase, MGC29949, Never in mitosis A-related kinase 3, NIMA (never in mitosis gene a)-related kinase 3, NimA-related protein kinase 3, phosphorylase B kinase kinase, serine/threonine-protein kinase Nek3

Entrez Gene IDs

4752 (Human)

Gene Symbol

NEK3

OMIM

604044 (Human)

UniProt

AAH19916 (Human)

Additional NEK3 Products

Product Documents for NEK3 Antibody (2F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEK3 Antibody (2F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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