NEK6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58672

Novus Biologicals, part of Bio-Techne
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NBP2-58672
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEK6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PVHPPDPQRHPNTLSFRCSLADFQIE

Reactivity Notes

Mouse 88%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEK6 Antibody

Western Blot: NEK6 Antibody [NBP2-58672]

Western Blot: NEK6 Antibody [NBP2-58672]

Western Blot: NEK6 Antibody [NBP2-58672] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: NEK6 Antibody [NBP2-58672]

Immunocytochemistry/ Immunofluorescence: NEK6 Antibody [NBP2-58672]

Immunocytochemistry/Immunofluorescence: NEK6 Antibody [NBP2-58672] - Staining of human cell line HeLa shows localization to nucleoplasm & cytosol.

Applications for NEK6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEK6

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

Alternate Names

EC 2.7.11.1, Never in mitosis A-related kinase 6, NIMA (never in mitosis gene a)-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512, putative serine-threonine protein kinase, serine/threonine-protein kinase Nek6, SID6-1512

Gene Symbol

NEK6

Additional NEK6 Products

Product Documents for NEK6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEK6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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