NEK7 Antibody (HL1348) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25575
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Predicted:
Chicken (93%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1348
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for NEK7 Antibody (HL1348) - Azide and BSA Free
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NEK7. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (86%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NEK7 Antibody (HL1348) - Azide and BSA Free
Knockout Validated: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] -
Knockout Validated: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] -
Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] -
Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for NEK7 Antibody (HL1348) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NEK7
Alternate Names
EC 2.7.11.1, Never in mitosis A-related kinase 7, NIMA (never in mitosis gene a)-related kinase 7, NimA-related protein kinase 7, serine/threonine-protein kinase Nek7
Gene Symbol
NEK7
Additional NEK7 Products
Product Documents for NEK7 Antibody (HL1348) - Azide and BSA Free
Product Specific Notices for NEK7 Antibody (HL1348) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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