NEK7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52115
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NTLANFRIEKK corresponding to internal region according to NP_598001.1.
Epitope
C-NTLANFRIEKK
Reactivity Notes
Expected from sequence similarity: Mouse, Rat, Canine, Bovine
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NEK7 Antibody
![Western Blot: NEK7 Antibody [NBP1-52115]](https://resources.bio-techne.com/images/products/NEK7-Antibody-Western-Blot-NBP1-52115-img0003.jpg "Western Blot: NEK7 Antibody [NBP1-52115]")
Western Blot: NEK7 Antibody [NBP1-52115]
Western Blot: NEK7 Antibody [NBP1-52115] - (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-52115]](https://resources.bio-techne.com/images/products/NEK7-Antibody-Immunohistochemistry-Paraffin-NBP1-52115-img0004.jpg "Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-52115]")
Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-52115]
Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-52115] - 3.75ug/ml staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for NEK7 Antibody
Application
Recommended Usage
Immunohistochemistry
0.05-0.2ug/ml
Peptide ELISA
Detection limit 1:64000
Western Blot
0.05 ug/ml
Application Notes
Western blot: Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Thyroid.
IHC: Paraffin embedded Human Thyroid.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NEK7
Alternate Names
EC 2.7.11.1, Never in mitosis A-related kinase 7, NIMA (never in mitosis gene a)-related kinase 7, NimA-related protein kinase 7, serine/threonine-protein kinase Nek7
Gene Symbol
NEK7
UniProt
Additional NEK7 Products
Product Documents for NEK7 Antibody
Product Specific Notices for NEK7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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