The molecular size of NEK8 is 75kDa. If you look at the WB image on the web for NBP1-82539(NEK8 antibody), the band is located between 70-100kDa (Closer to 100kDa). In our WB testing, we also observed the band near 100kDa. Do you think that it is the band of NEK8?

Name: NEK8 Antibody
Brand: Novus Biologicals
SKU: NBP1-82539

The predicted size of NEK8 protein is 75 kDa. However, the actual observed size of the protein is dependent on the sample type. If the protein has undergone post-translational modifications, then the size of the protein band could be higher or lower than the predicted one. Without a positive control such as a purified protein or overexpressed lysate, it is very hard to confirm 100% whether the band is real or not. We have not specifically tested MCF7 cell line, however we suspect that the band observed by you is the post-translationally modified NEK8 protein running at higher than predicted molecular weight.

NEK8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82539

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEK8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%), Mouse (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEK8 Antibody

Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539]

Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Applications for NEK8 Antibody

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEK8

NEK8 (NIMA-Related Kinase 8) belongs to the protein kinase superfamily. Mutations in NEK8 have been linked to nephronophthisis type 9. NEK8 is required for renal tubular integrity. NEK8 is known to have interactions with ABL1, FYN, ACVR1, BICD2 and CSN2.

Alternate Names

EC 2.7.11, EC 2.7.11.1, JCKNIMA-related kinase 12a, MGC138445, NEK12A, nephrocystin 9, Never in mitosis A-related kinase 8, NIMA (never in mitosis gene a)- related kinase 8, Nima-related protein kinase 12a, NimA-related protein kinase 8, NPHP9NIMA-family kinase NEK8, serine/threonine-protein kinase Nek8, serine/thrionine-protein kinase NEK8

Gene Symbol

NEK8

Additional NEK8 Products

Product Documents for NEK8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for NEK8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NEK8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82539

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all NEK8 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Scientific Data Images for NEK8 Antibody

Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539]

Applications for NEK8 Antibody

Immunohistochemistry

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: NEK8

Alternate Names

Gene Symbol

Additional NEK8 Products

Product Documents for NEK8 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for NEK8 Antibody

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