NEK9 Antibody (OTI1H1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01070
![Western Blot: NEK9 Antibody (OTI1H1) [NBP2-01070]](https://resources.bio-techne.com/images/products/NEK9-Antibody-1H1-Western-Blot-NBP2-01070-img0002.jpg "Western Blot: NEK9 Antibody (OTI1H1) [NBP2-01070]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1H1
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human NEK9(NP_149107) produced in HEK293 cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NEK9 Antibody (OTI1H1)
Western Blot: NEK9 Antibody (OTI1H1) [NBP2-01070]
Western Blot: NEK9 Antibody (1H1) [NBP2-01070] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.
Immunocytochemistry/Immunofluorescence: NEK9 Antibody (1H1) [NBP2-01070] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.
![Flow Cytometry: NEK9 Antibody (OTI1H1) [NBP2-01070]](https://resources.bio-techne.com/images/products/NEK9-Antibody-1H1-Flow-Cytometry-NBP2-01070-img0001.jpg "Flow Cytometry: NEK9 Antibody (OTI1H1) [NBP2-01070]")
Flow Cytometry: NEK9 Antibody (OTI1H1) [NBP2-01070]
Flow Cytometry: NEK9 Antibody (1H1) [NBP2-01070] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK9 antibody, and then analyzed by flow cytometry.Applications for NEK9 Antibody (OTI1H1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NEK9
Long Name
Serine/threonine-protein kinase Nek9
Alternate Names
KIAA1995, Nek8, NERCC, Nercc1 kinase
Entrez Gene IDs
91754 (Human)
Gene Symbol
NEK9
Additional NEK9 Products
Product Documents for NEK9 Antibody (OTI1H1)
Product Specific Notices for NEK9 Antibody (OTI1H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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