NELF-E Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88036

Novus Biologicals, part of Bio-Techne
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NBP1-88036
NBP1-88036-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NELF-E Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NELF-E Antibody

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036]

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036]

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036] - Staining in human testis and skeletal muscle tissues . Corresponding NELFE RNA-seq data are presented for the same tissues.
Western Blot: NELF-E Antibody [NBP1-88036]

Western Blot: NELF-E Antibody [NBP1-88036]

Western Blot: NELF-E Antibody [NBP1-88036] - Analysis using Anti-NELFE antibody NBP1-88036 (A) shows similar pattern to independent antibody NBP2-55487 (B).
Western Blot: NELF-E Antibody [NBP1-88036]

Western Blot: NELF-E Antibody [NBP1-88036]

Western Blot: NELF-E Antibody [NBP1-88036] - Analysis in human cell line MOLT-4.
View 4 more images

Applications for NELF-E Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NELF-E

NELF-E is encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq]

Alternate Names

D6S45, negative elongation factor E, negative elongation factor polypeptide E, NELFE, NELF-ERDmajor histocompatibility complex gene RD, nuclear protein, RD RNA binding protein, RD RNA-binding protein, RDP, RNA-binding protein RD

Gene Symbol

NELFE

Additional NELF-E Products

Product Documents for NELF-E Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NELF-E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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