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Neprilysin/CD10 Antibody (MME/6696R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20611

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MME/6696R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant human Neprilysin/CD10 protein fragment (aa 1-500) (exact sequence is proprietary)

Localization

Cell surface. Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Tonsil, small intestine or kidney.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.

Scientific Data Images for Neprilysin/CD10 Antibody (MME/6696R)

Neprilysin/CD10 Antibody (MME/6696R)

Neprilysin/CD10 Antibody (MME/6696R)

Formalin-fixed, paraffin-embedded human kidney stained with Neprilysin/CD10 antibody (MME/6696R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Neprilysin/CD10 Antibody (MME/6696R)

Neprilysin/CD10 Antibody (MME/6696R)

Formalin-fixed, paraffin-embedded human prostate stained with Neprilysin/CD10 antibody (MME/6696R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Neprilysin/CD10 Antibody (MME/6696R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Neprilysin/CD10

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Gene Symbol

MME

Additional Neprilysin/CD10 Products

Product Documents for Neprilysin/CD10 Antibody (MME/6696R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neprilysin/CD10 Antibody (MME/6696R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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