Nesprin 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42886
![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886]](https://resources.bio-techne.com/images/products/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-42886-img0007.jpg "Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse Nesprin 2. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
783 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nesprin 2 Antibody
![Western Blot: Nesprin 2 Antibody [NBP2-42886]](https://resources.bio-techne.com/images/products/Nesprin-2-Antibody-Western-Blot-NBP2-42886-img0006.jpg "Western Blot: Nesprin 2 Antibody [NBP2-42886]")
Western Blot: Nesprin 2 Antibody [NBP2-42886]
Western Blot: Nesprin 2 Antibody [NBP2-42886] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nesprin 2 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886]](https://resources.bio-techne.com/images/products/Nesprin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-42886-img0008.jpg "Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886]")
Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886]
Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] - Rat brain. Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Immunohistochemistry-Frozen: Nesprin 2 Antibody [NBP2-42886]](https://resources.bio-techne.com/images/products/Nesprin-2-Antibody-Immunohistochemistry-Frozen-NBP2-42886-img0004.jpg "Immunohistochemistry-Frozen: Nesprin 2 Antibody [NBP2-42886]")
Immunohistochemistry-Frozen: Nesprin 2 Antibody [NBP2-42886]
Immunohistochemistry-Frozen: Nesprin 2 Antibody [NBP2-42886] - Frozen sectioned adult mouse retina. Green: Nesprin 2 protein stained by Nesprin 2 antibody [C3], C-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:250. Blue: Fluoroshield with DAPI.Applications for Nesprin 2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
-Assay dependent
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nesprin 2
Alternate Names
DKFZp434H2235, DKFZp686E01115, DKFZp686H1931, EDMD5, FLJ46790, KIAA1011FLJ11014, nesprin 2, Nesprin-2, NUAFLJ43727, NUANCEFLJ45710, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, polytrophin, Protein NUANCE, spectrin repeat containing, nuclear envelope 2, Synaptic nuclear envelope protein 2, synaptic nuclei expressed gene 2, Syne-2, TROPH
Gene Symbol
SYNE2
Additional Nesprin 2 Products
Product Documents for Nesprin 2 Antibody
Product Specific Notices for Nesprin 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.