Nestin Antibody

Western Blot: Nestin Antibody [NB100-1604]

20 ug of total protein from adult mouse brain using NB100-1604 (1:2000)

Immunohistochemistry: Nestin Antibody [NB100-1604]

Photomicrograph of a tissue section through the periventricular zone of an e16 mouse brain (4.0% paraformaldehyde-fixed). Green is Nestin immunoreactivity (1:1000 dilution). Blue is DAPI nuclear staining.

Immunohistochemistry: Nestin Antibody [NB100-1604] -

Representative images of nestin (A) and Tmsb4x (B) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (C) Merged images showing Tmsb4x (red) and nestin (green) staining; areas of colocalization are indicated by arrows. Representative images of Tmsb4x (D) and Cd31 (E) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (F) Merged images showing Tmsb4x (green) and Cd31 (red) staining. Bar = 20 μm.

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] -

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - In vitro proliferation & differentiation of ahMNCs-Clump II. (A) ahMNCs-Clump II were propagated in the culture condition for ahMNCs. The accumulated number of cells of ahMNCs-Clump II (NS18-007TL & 008TL) in comparison with ahMNCs established using previous culture methods (NS14-010TL, NS14-011TL, & NS15-001TL). Days to 1 × 109 cells are indicated. (B) Morphologies of ahMNCs-Clump II under expansion processes are illustrated until third in vitro passage (P3). Scale bar = 50 μm. (C) After in vitro differentiation, immunofluorescence was applied to ahMNCs-Clump II. Nestin for NSCs, MAP2 & Tuj1 for neurons, & GFAP for astrocytes. Undiffer = before in vitro differentiation; Differ = after in vitro differentiation. Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30380605), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] -

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Representative images of nestin (A) & Tmsb4x (B) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (C) Merged images showing Tmsb4x (red) & nestin (green) staining; areas of colocalization are indicated by arrows. Representative images of Tmsb4x (D) & Cd31 (E) staining in the mouse adult wild-type glomerulus visualized by fluorescent microscopy. (F) Merged images showing Tmsb4x (green) & Cd31 (red) staining. Bar = 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27575556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] -

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Dopaminergic neuron derivation from H9 stem cells & hIPSCs.Scheme showing the different steps of the DA neuron derivation protocol (A). Immunolabelings of cultured H9 (top panels) & WT hiPSCs (bottom panels) for different NSCs markers, Sox1, Pax6 & Nestin (B). Immunolabeling of cultured H9 (top panels) & WT hiPSCs (bottom panels) after 3 days of DA progenitor induction for progenitor markers Lmx1A & FoxA2 (C) & after 7 days of induction for immature neurons betaIII-Tubulin+ & remaining progenitors Sox1+ (D). Immunolabelings of cultured H9 (top panels) & WT hiPSCs (bottom panels) after 21 days of induction for the neuronal marqueur MAP2 & DA neurons Tyrosine Hydroxylase (TH) (E). Analysis of gene expression levels by qRT-PCR for cultured H9 (top panels) & WT hiPSCs at different time points (NSC, 14 days & 21 days) of the culture (FB = fetal brain) (F,G). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep33377), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] -

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - Neural Stem Cell derivation from WT & LRRK2 G2019S hiPSCs.Haematoxylin & eosin coloration of teratoma-encapsulated tumors generated by flank injection of WT & G2019S hiPSCs cells in Nod/Scid mice. Representative images of the different germ layers: ectoderm (immature squamous epithelium, neural rosettes), mesoderm (primitive cartilage, muscles, fat) & endoderm (primitive gut like epithelium) (A). Scheme showing the different steps of the DA neuron derivation protocol (B). Immunolabelings of cultured WT & LRRK2 G2019S hIPSCs for different NSCs markers after NSCs induction protocol: Sox1, Nestin, Pax6 & Ki67 (C). Analysis of gene expression levels by qRT-PCR for WT & LRRK2 G2019S cultures at the end of the NSCs induction protocol (D). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep33377), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] -

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB100-1604] - PAR1 is expressed by neural stem cells in the sub-ventricular zone (SVZ) of the adult mouse brain. Photomicrographs show immunofluorescence double-labeling for PAR1 with Sox2-positive (A), or PAR1 with Nestin-positive (C) multipotent neural stem cells (NSCs) within the lateral wall of the lateral ventricle (LV). RNAscope was used to identify cells expressing both PAR1 & Sox2 (B), or Nestin (D) RNA in NSCs of the adult SVZ. Arrow indicates an example of a double-labeled cell in each case, with arrowhead indicating a singly labeled cell (Scale bar = 10 μm). Boxed area in B & D is also shown at higher magnification to visualize double-labeled cells. (E) Histogram shows expression of PAR1 RNA was high in NSCs grown as neurospheres (NS), or when plated on poly-L-lysine coated coverslips as monolayers in stem cell media containing EGF & bFGF. PAR1 RNA expression by NSC monolayers decreased by 87% when EGF & bFGF were removed from the media for 7 DIV promoting stem cell differentiation. (F) Withdrawal of EGF & bFGF to induce NSC monolayer differentiation resulted in a parallel decrease in Nestin RNA expression. (**P < 0.01, ***P < 0.001 Students t-test). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29921916), licensed under a CC-BY license. Not internally tested by Novus Biologicals.